Anti-DOK7 抗体 (ab75049)
Key features and details
- Rabbit polyclonal to DOK7
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-DOK7 antibody -
製品の詳細
Rabbit polyclonal to DOK7 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide derived from N-terminus of human DOK7.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab75049の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
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IHC-P |
1/50 - 1/100.
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ICC/IF |
1/500 - 1/1000.
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特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa). |
IHC-P
1/50 - 1/100. |
ICC/IF
1/500 - 1/1000. |
ターゲット情報
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機能
Probable muscle-intrinsic activator of MUSK that plays an essential role in neuromuscular synaptogenesis. Acts in aneural activation of MUSK and subsequent acetylcholine receptor (AchR) clustering in myotubes. Induces autophosphorylation of MUSK. -
組織特異性
Preferentially expressed in skeletal muscle and heart Present in thigh muscle, diaphragm and heart but not in the liver or spleen (at protein level). -
関連疾患
Defects in DOK7 are the cause of familial limb-girdle myasthenia autosomal recessive (LGM) [MIM:254300]; also called congenital myasthenic syndrome type 1B or CMS1B. LGM is a congenital myasthenic syndrome characterized by a typical 'limb girdle' pattern of muscle weakness with small, simplified neuromuscular junctions but normal acetylcholine receptor and acetylcholinesterase function. -
配列類似性
Contains 1 IRS-type PTB domain.
Contains 1 PH domain. -
細胞内局在
Cell membrane. Cell junction > synapse. Accumulates at neuromuscular junctions. - Information by UniProt
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参照データベース
- Entrez Gene: 285489 Human
- Entrez Gene: 231134 Mouse
- SwissProt: Q18PE1 Human
- SwissProt: Q18PE0 Mouse
- Unigene: 122110 Human
- Unigene: 701584 Human
- Unigene: 205483 Mouse
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別名
- Docking protein 7 antibody
- DOK 7 antibody
- DOK7 antibody
see all
画像
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All lanes : Anti-DOK7 antibody (ab75049) at 1/500 dilution
Lane 1 : extracts from mouse brain cells
Lane 2 : extracts from mouse brain cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 53 kDa
Observed band size: 53 kDa
Additional bands at: 46 kDa, 80 kDa. We are unsure as to the identity of these extra bands. -
Human brain tissue with ab75049 at 1/50 dilution, in the absence and presence of the immunising peptide.
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HepG2 cells with ab75049 at 1/500 dilution, in the absence and presence of the immunising peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab75049 は 3 報の論文で使用されています。
- Yue C et al. DOK7 Inhibits Cell Proliferation, Migration, and Invasion of Breast Cancer via the PI3K/PTEN/AKT Pathway. J Oncol 2021:4035257 (2021). PubMed: 33552156
- Huot JR et al. Muscle weakness caused by cancer and chemotherapy is associated with loss of motor unit connectivity. Am J Cancer Res 11:2990-3001 (2021). PubMed: 34249440
- Zhao H et al. DOK7V1 influences the malignant phenotype of lung cancer cells through PI3K/AKT/mTOR and FAK/paxillin signaling pathways. Int J Oncol 54:381-389 (2019). PubMed: 30431081