Anti-DNAJB6 抗体 (ab96539)
Key features and details
- Rabbit polyclonal to DNAJB6
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-DNAJB6 antibody
DNAJB6 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DNAJB6 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow -
免疫原
Recombinant protein fragment with a sequence corresponding to a region within amino acids 1 and 126 of Human DNAJB6 (NP_490647).
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ポジティブ・コントロール
- HeLaS3 and MOLT4 cell lysates for WB and HeLa cells for IF
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96539の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 36 kDa.
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ICC/IF |
1/100 - 1/200.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 36 kDa. |
ICC/IF
1/100 - 1/200. |
ターゲット情報
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機能
Plays an indispensable role in the organization of KRT8/KRT18 filaments. Acts as an endogenous molecular chaperone for neuronal proteins including huntingtin. Suppresses aggregation and toxicity of polyglutamine-containing, aggregation-prone proteins. Isoform B but not isoform A inhibits huntingtin aggregation. Has a stimulatory effect on the ATPase activity of HSP70 in a dose-dependent and time-dependent manner and hence acts as a co-chaperone of HSP70. Also reduces cellular toxicity and caspase-3 activity. -
組織特異性
Widely expressed. Highest levels in testis and brain, and lower levels in heart, spleen, intestine, ovary, placenta, lung, kidney, pancreas, thymus, prostate, skeletal muscle, liver and leukocytes. In testis, expressed in germ cells in the earlier stages of differentiation pathway as well as in spermatids. In brain, expressed at a higher level in hippocampus and thalamus and a lower level in amygdala, substantia nigra, corpus callosum and caudate nucleus. -
関連疾患
Limb-girdle muscular dystrophy 1E -
配列類似性
Contains 1 J domain. -
ドメイン
The antiaggregation activity of isoform B resides in the serine-rich region and the C-terminus. -
細胞内局在
Cytoplasm, perinuclear region. Nucleus. Cytoplasm, myofibril, sarcomere, Z line. - Information by UniProt
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参照データベース
- Entrez Gene: 282215 Cow
- Entrez Gene: 10049 Human
- Entrez Gene: 23950 Mouse
- Entrez Gene: 362293 Rat
- Omim: 611332 Human
- SwissProt: Q0III6 Cow
- SwissProt: O75190 Human
- SwissProt: O54946 Mouse
see all -
別名
- DJ4 antibody
- DnaJ antibody
- DnaJ (Hsp40) homolog subfamily B member 6 antibody
see all
画像
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab96539 は 3 報の論文で使用されています。
- Qian FY et al. A novel recessive mutation affecting DNAJB6a causes myofibrillar myopathy. Acta Neuropathol Commun 9:23 (2021). PubMed: 33557929
- Palmio J et al. Mutations in the J domain of DNAJB6 cause dominant distal myopathy. Neuromuscul Disord 30:38-46 (2020). PubMed: 31955980
- Ghaoui R et al. Mutations in HSPB8 causing a new phenotype of distal myopathy and motor neuropathy. Neurology 86:391-8 (2016). PubMed: 26718575