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Western blot - DNA polymerase alpha antibody (ab31777)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DNA polymerase alpha antibody (ab31777)
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Immunocytochemistry/ Immunofluorescence - DNA polymerase alpha antibody (ab31777)
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Product Name
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DNA polymerase alpha antibody
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DNA polymerase alpha 抗体 (3件) 一覧
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to DNA polymerase alpha
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Immunogen
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Synthetic peptide derived from within residues 1100 - 1200 of Human DNA polymerase alpha.
(Peptide available as ab30406.)
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Reacts with
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Hu, Chk
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Predicted to react
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Ms, Rat, Dog, Xl
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-P: 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 166 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> DNA Polymerases
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Relevance
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DNA polymerase alpha can be part of a complex with DNA primase and act as a replicative polymerase. In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon. Each of them is responsible for a different reaction during DNA synthesis.
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DNA polymerase alpha antibody (ab31777)の別名 |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.20 - 0.27 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Abcam では全ての製品情報を一元管理しており、 DNA polymerase alpha antibody について私たちが知りうる全ての情報がこのデータシートでご覧いただけます。ご不明な点がありましたら、 お問い合わせください。 |
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See below for DNA polymerase alpha antibody images, references, products related to ab31777 and other tools.
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DNA polymerase alpha antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DNA polymerase alpha antibody (ab31777)
Image courtesy of Human Protein Atlas ab31777 staining DNA polymerase alpha in human thyroid gland, showing a strong staining in nuclei of the glandular cells. Paraffin embedded human thyroid tissue was incubated with ab31777 (1/100 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab31777 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
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Immunocytochemistry/ Immunofluorescence - DNA polymerase alpha antibody (ab31777)
ICC/IF image of ab31777 stained human MCF7 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab31777, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HEK 293 and HepG2 cells.
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Search PubMed (MEDLINE) for references to DNA polymerase alpha
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DNA polymerase alpha antibody - more information
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Customer reviews (feedback) regarding DNA polymerase alpha antibody |
Customer FAQs regarding DNA polymerase alpha antibody |
Protocols for DNA polymerase alpha antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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