Application
Western blot
Sample
Human Cell lysate - whole cell (1 EBNA, 2 HeLa)
Loading amount
15 µg
Specification
1 EBNA, 2 HeLa
Gel Running Conditions
Reduced Denaturing
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 2%
Other product details
Dilution
1/500
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 12°C · Diluent: ECL Advance Blocking Solution
Secondary antibody
Name
Non-Abcam antibody was used: Donkey-anti-rabbit HRP
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/100000
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 42 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Dec 16 2008