Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Permeabilization
Yes - 0.5% Triton X100 in PBS
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-mouse AlexaFluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200
Additional data
Additional Notes
Asynchronous cells were bleomycin treated, paraformaldehyde fixed, permeablized, immunofluorescently labeled and counterstained with DAPI. The antibody produces a weak nuclear-enriched staining pattern but does not appear to give a DNA DSB pattern. MeOH fixed samples were also evaluated and failed to produce the expected staining pattern.
Abcam response
Thank you for your valuable feedback. Your data suggests that ab11054 may not be suitable to detect endogenous DMC1 by ICC in HeLa cells under these experimental conditions. We hope this information is useful to our customers and we welcome further feedback.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kirk Mcmanus
Verified customer
投稿 Nov 15 2013