Anti-DKC1/Dyskerin 抗体 (ab93777)
Key features and details
- Rabbit polyclonal to DKC1/Dyskerin
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-DKC1/Dyskerin antibody
DKC1/Dyskerin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DKC1/Dyskerin -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Drosophila melanogaster, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis -
免疫原
Synthetic peptide corresponding to Human DKC1/Dyskerin aa 340-390.
Database link: NP_001354.1 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab93777の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 58 kDa.
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IP |
Use at 5-15 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 58 kDa. |
IP
Use at 5-15 µg/mg of lysate. |
ターゲット情報
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機能
Isoform 1: Required for ribosome biogenesis and telomere maintenance. Probable catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ('psi') residues, which may serve to stabilize the conformation of rRNAs. Also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.
Isoform 3: Promotes cell to cell and cell to substratum adhesion, increases the cell proliferation rate and leads to cytokeratin hyper-expression (when overexpressed in HeLa cells). -
組織特異性
Ubiquitously expressed. -
関連疾患
Defects in DKC1 are a cause of dyskeratosis congenita X-linked recessive (XDKC) [MIM:305000]. XDKC is a rare, progressive bone marrow failure syndrome characterized by the triad of reticulated skin hyperpigmentation, nail dystrophy, and mucosal leukoplakia. Early mortality is often associated with bone marrow failure, infections, fatal pulmonary complications, or malignancy.
Defects in DKC1 are the cause of Hoyeraal-Hreidarsson syndrome (HHS) [MIM:300240]. HHS is a multisystem disorder affecting males and is characterized by aplastic anemia, immunodeficiency, microcephaly, cerebellar hypoplasia, and growth retardation. -
配列類似性
Belongs to the pseudouridine synthase TruB family.
Contains 1 PUA domain. -
細胞内局在
Cytoplasm and Nucleus > nucleolus. Nucleus > Cajal body. Also localized to Cajal bodies. - Information by UniProt
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参照データベース
- Entrez Gene: 37873 Drosophila melanogaster
- Entrez Gene: 1736 Human
- Entrez Gene: 245474 Mouse
- Entrez Gene: 170944 Rat
- Omim: 300126 Human
- SwissProt: O44081 Drosophila melanogaster
- SwissProt: O60832 Human
- SwissProt: Q9ESX5 Mouse
see all -
別名
- CBF5 antibody
- CBF5 homolog antibody
- Cbf5p homolog antibody
see all
画像
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All lanes : Anti-DKC1/Dyskerin antibody (ab93777) at 0.04 µg/ml
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 58 kDa
Exposure time: 3 minutes -
Detection of Human DKC1/Dyskerin by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded)using ab93777 at 10 ug/mg lysate (LANE 1). DKC1/Dyskerin was also immunoprecipitated by an antibody which recognizes a downstream epitope (LANE 2). Lane 3 represents IgG Ip control. subsequent WB detection was performed using 1 ug/ml ab93777. Detection: Chemiluminescence with exposure times of 10 seconds.
All lanes : Anti-DKC1/Dyskerin antibody (ab93777) at 1 µg/ml
Lane 1 : HeLa cell lysate immunoprecipitated with ab 93777 at 10ug/mg lysate.
Lane 2 : HeLa cell lysate immunoprecipitated with anti-DKC1/Dyskerinantibody at 10ug/mg lysate.
Lane 3 : HeLa cell lysate immunoprecipitated with control IgG at 10ug/mg lysate.
Lysates/proteins at 200 µg per lane.
Developed using the ECL technique.
Exposure time: 10 seconds
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab93777 は 3 報の論文で使用されています。
- Yang Z et al. LncRNA MEG3 inhibits non-small cell lung cancer via interaction with DKC1 protein. Oncol Lett 20:2183-2190 (2020). PubMed: 32782535
- Yao RW et al. Nascent Pre-rRNA Sorting via Phase Separation Drives the Assembly of Dense Fibrillar Components in the Human Nucleolus. Mol Cell 76:767-783.e11 (2019). PubMed: 31540874
- Xing YH et al. SLERT Regulates DDX21 Rings Associated with Pol I Transcription. Cell 169:664-678.e16 (2017). PubMed: 28475895