Application
Immunocytochemistry/ Immunofluorescence
Sample
Fruit fly (Drosophila melanogaster) Cell (Ovary and testie)
Permeabilization
Yes - PBST
Specification
Ovary and testie
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Other product details
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/200
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Cy5 conjugated AffiniPure Donkey Anti Rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy5 ®
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy5 ®
Additional data
Additional Notes
The w1118 pictures are wild type and the CG9323 pictures should show no expression of DHX36. I do not think it is a great antibody, there is a lot of background and it seems to be staining the somatic cells as well.
Abcam response
Thank you for your review of this untested species. We will continue to collect feedback on use in Drosophila.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 May 02 2016