Anti-DHX30 抗体 (ab85754)
Key features and details
- Rabbit polyclonal to DHX30
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-DHX30 antibody
DHX30 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DHX30 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan -
免疫原
Synthetic peptide, corresponding to a region between residues 1144 and 1194 of human DHX30 (NP_619520.1)
-
ポジティブ・コントロール
- HeLa and 293T whole cell lysates.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
-
精製度
Immunogen affinity purified -
特記事項(精製)
ab85754 was affinity purified using an epitope specific to DHX30 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab85754の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/2000 - 1/10000. Detects a band of approximately 134 kDa (predicted molecular weight: 134 kDa).
|
|
IP |
Use at 5-10 µg/mg of lysate.
|
特記事項 |
---|
WB
1/2000 - 1/10000. Detects a band of approximately 134 kDa (predicted molecular weight: 134 kDa). |
IP
Use at 5-10 µg/mg of lysate. |
ターゲット情報
-
配列類似性
Belongs to the DEAD box helicase family. DEAH subfamily.
Contains 1 DRBM (double-stranded RNA-binding) domain.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
翻訳後修飾
Isoform 2 is phosphorylated on Ser-15. -
細胞内局在
Cytoplasm and Mitochondrion. Cytoplasm. Mitochondrion matrix > mitochondrion nucleoid. - Information by UniProt
-
参照データベース
- Entrez Gene: 22907 Human
- Entrez Gene: 367172 Rat
- GenBank: NM_138614 Human
- SwissProt: Q7L2E3 Human
- SwissProt: Q7L2E3-2 Human
- SwissProt: Q5BJS0 Rat
- Unigene: 517948 Human
- Unigene: 163034 Rat
-
別名
- DDX30 antibody
- DEAH box protein 30 antibody
- Dhx30 antibody
see all
画像
-
All lanes : Anti-DHX30 antibody (ab85754) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 134 kDa
Observed band size: 134 kDa
Additional bands at: 110 kDa, 180 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
Detection of Human DHX3 by Western Blot of Immunprecipitate.
All lanes: ab85754 at 0.4µg/ml staining DHX3 in HeLa whole cell lysates immunoprecipitated at 10µg/mg lysate (1 mg/IP; 20% of IP loaded/lane) using the following antibodies:
Lane 1: ab85754
Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 3 minutes.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab85754 は論文での使用が確認できていません。