DHEA sulfate (DHEA-S) ELISA Kit (ab108669)
Key features and details
- Sensitivity: 0.04 ng/ml
- Range: 0.1 µg/ml - 10 µg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
製品の概要
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製品名
DHEA sulfate (DHEA-S) ELISA Kit
DHEA-S キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Control 64 < 7.9% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Control 48 < 10.4% -
サンプルの種類
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
アッセイタイプ
Competitive -
検出感度
= 0.04 ng/ml -
検出範囲
0.1 µg/ml - 10 µg/ml -
添加回収試験
= 96.71 %
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ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s DHEA sulfate (DHEA-S) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of DHEA sulfate in plasma and serum.
A 96-well plate has been precoated with anti-DHEA sulphate antibodies. Samples and the DHEA sulfate-HRP conjugate are added to the wells, where DHEA sulfate in the sample competes with the added DHEA sulfate-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of DHEA sulfate in the sample and the intensity is measured at 450 nm.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Wash Solution 1 x 50ml Serum Diluent 1 x 100ml Anti-DHEA sulfate antibody Coated Microplate (12 x 8 wells) 1 unit Control A 1 x 1ml Control B 1 x 1ml Cover foils 1 unit DHEA sulfate Standard 0 – 0 µg/mL 1 x 1ml DHEA sulfate Standard 1 – 0.1 µg/mL 1 x 1ml DHEA sulfate Standard 2 – 0.4 µg/mL 1 x 1ml DHEA sulfate Standard 3 – 1.0 µg/mL 1 x 1ml DHEA sulfate Standard 4 – 4.0 µg/mL 1 x 1ml DHEA sulfate Standard 5 – 10.0 µg/mL 1 x 1ml DHEA sulfate-HRP Conjugate 1 x 12ml Stop Solution 1 x 15ml Strip holder 1 unit TMB Substrate Solution 1 x 15ml -
研究分野
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab108669 は 4 報の論文で使用されています。
- Wen M et al. CTBP1‑AS upregulation is associated with polycystic ovary syndrome and can be effectively downregulated by cryptotanshinone. Mol Med Rep 26:N/A (2022). PubMed: 35656892
- Li IC et al. Prevention of Early Alzheimer's Disease by Erinacine A-Enriched Hericium erinaceus Mycelia Pilot Double-Blind Placebo-Controlled Study. Front Aging Neurosci 12:155 (2020). PubMed: 32581767
- Martínez de Toda I et al. High perceived stress in women is linked to oxidation, inflammation and immunosenescence. Biogerontology 20:823-835 (2019). PubMed: 31396798
- Grimby-Ekman A et al. Different DHEA-S Levels and Response Patterns in Individuals with Chronic Neck Pain, Compared with a Pain Free Group-a Pilot Study. Pain Med N/A:N/A (2016). Competitive ELISA ; Human . PubMed: 27498549