Anti-DGKA 抗体 (ab88672)
Key features and details
- Mouse polyclonal to DGKA
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-DGKA antibody
DGKA 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to DGKA -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human DGKA. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ・コントロール
- Human spleen lysate; Hela cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab88672の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 83 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 83 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity. -
組織特異性
Lymphocytes and oligodendroglial cells. -
配列類似性
Belongs to the eukaryotic diacylglycerol kinase family.
Contains 1 DAGKc domain.
Contains 2 EF-hand domains.
Contains 2 phorbol-ester/DAG-type zinc fingers. - Information by UniProt
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参照データベース
- Entrez Gene: 1606 Human
- Omim: 125855 Human
- SwissProt: P23743 Human
- Unigene: 524488 Human
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別名
- 80 kDa diacylglycerol kinase antibody
- DAG kinase alpha antibody
- DAGK antibody
see all
画像
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Anti-DGKA antibody (ab88672) at 1 µg/ml + Human spleen lysate at 50 µg
Predicted band size: 83 kDa -
All lanes : Anti-DGKA antibody (ab88672) at 1 µg/ml
Lane 1 : DGKA transfected 293T cell lysate
Lane 2 : non transfected lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 83 kDa
Observed band size: 81 kDa why is the actual band size different from the predicted? -
Immunofluorescence staining of DGKA in HeLa cells using ab88672 at 10 µg/ml.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (1)
ab88672 は 1 報の論文で使用されています。
- Lupino E et al. I?B kinase ß is required for activation of NF-?B and AP-1 in CD3/CD28-stimulated primary CD4(+) T cells. J Immunol 188:2545-55 (2012). Human . PubMed: 22331067