Anti-DGAT1 抗体 (ab59034)
Key features and details
- Goat polyclonal to DGAT1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-DGAT1 antibody
DGAT1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to DGAT1 -
由来種
Goat -
アプリケーション
適用あり: IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Goat, Cow, Dog, Pig, African green monkey -
免疫原
Synthetic peptide:
C-QNSMKPFKDMDYS
, corresponding to internal sequence amino acids 305-317 of Human DGAT1 -
ポジティブ・コントロール
- WB: Human duodenum and mouse liver tissue lysates. ICC/IF: A431 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab59034の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
Use a concentration of 3.75 µg/ml.
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WB | (2) |
Use a concentration of 0.01 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
A 1 hour primary incubation is recommended for this product. |
ICC/IF |
Use a concentration of 10 µg/ml.
|
特記事項 |
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IHC-P
Use a concentration of 3.75 µg/ml. |
WB
Use a concentration of 0.01 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). A 1 hour primary incubation is recommended for this product. |
ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly. -
パスウェイ
Lipid metabolism; glycerolipid metabolism. -
配列類似性
Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily. -
細胞内局在
Endoplasmic reticulum membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 282609 Cow
- Entrez Gene: 482093 Dog
- Entrez Gene: 8694 Human
- Entrez Gene: 13350 Mouse
- Entrez Gene: 397118 Pig
- Entrez Gene: 84497 Rat
- Omim: 604900 Human
- SwissProt: Q8MK44 Cow
see all -
別名
- ACAT related gene product 1 antibody
- ACAT-related gene product 1 antibody
- Acyl coenzyme A:cholesterol acyltransferase related gene 1 antibody
see all
画像
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immunofluorescence analysis of A431 cells labelling DGAT1 with ab59034 at 10 µg/mL or dilution]. Cells were permeabilized with 0.15% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 2 µg/mL was used as the secondary antibody (green). Nuclear DNA was labelled with DAPI (blue). Negative control: Unimmunized goat IgG 10 µg/mL. Strong expression of the protein seen in the endoplasmic reticulum and cytoplasm is seen.
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Anti-DGAT1 antibody (ab59034) at 0.03 µg/ml + Mouse liver lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 55 kDa
Observed band size: 55 kDaPrimary incubation: 1 hour at room temperature.
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Anti-DGAT1 antibody (ab59034) at 0.5 µg/ml + Human Duodenum lysate (35µg protein in RIPA buffer)
Predicted band size: 55 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
データシートおよび資料
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Datasheet download
参考文献 (5)
ab59034 は 5 報の論文で使用されています。
- Lv Y et al. Metabolic Transition of Milk Triacylglycerol Synthesis in Response to Varying Levels of Three 18-Carbon Fatty Acids in Porcine Mammary Epithelial Cells. Int J Mol Sci 22:N/A (2021). PubMed: 33525494
- Howie D et al. A Novel Role for Triglyceride Metabolism in Foxp3 Expression. Front Immunol 10:1860 (2019). PubMed: 31456800
- Soayfane Z et al. Exposure to dietary lipid leads to rapid production of cytosolic lipid droplets near the brush border membrane. Nutr Metab (Lond) 13:48 (2016). PubMed: 27478484
- Kaylor JJ et al. Diacylglycerol O-acyltransferase type-1 synthesizes retinyl esters in the retina and retinal pigment epithelium. PLoS One 10:e0125921 (2015). WB . PubMed: 25974161
- Buers I et al. TIP47, a lipid cargo protein involved in macrophage triglyceride metabolism. Arterioscler Thromb Vasc Biol 29:767-73 (2009). PubMed: 19286631