Application
Western blot
Sample
Cat Cell lysate - whole cell (Skin Fibroblasts and Vaccination Associated Sarcom)
Gel Running Conditions
Reduced Denaturing (12% Gel)
Loading amount
10 µg
Specification
Skin Fibroblasts and Vaccination Associated Sarcom
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in TBST
Dilution
1/5000
Detection
Detection method
ECL Select
Negative control
GAR-only staining control of same samples (image not included) showed nonspecific band at 42 kDa in all samples.
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 54, 100 kDa Non-specific: 42 kDa
Positive control
Human PC3 (Prostate Cancer Bone Metastasis) Cell Line (Lane 4).
Additional data
Additional Notes
Samples in lanes 1-4 stained with 1:5000 ab70455 and 1:40000 GAR
Lane 1 ladder: 250, 150, 100 , 75, 50 , 37 kDa.
Lane 2: Cat Skin Fibroblasts
Lane 3: Cat VAS Cell Line
Lane 4: Human PC3 Cell Line
Lane 5: Cat Skin Fibroblasts
Lane 6: Cat VAS Cell Line
Lane 7: Human PC3 Cell Line
Lanes 5-7 were stained with only the secondary, 1:40000 GAR.
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投稿 Nov 06 2017