製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to DDX52
  • アプリケーション
    適用あり: IPmore details
    適用なし: WB
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 49-549 of Human DDX52 (NP_008941.2)

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8.
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab112027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at 2-5 µg/mg of lysate.
  • 追加情報
    Is unsuitable for WB.
  • ターゲット情報

    • 配列類似性
      Belongs to the DEAD box helicase family. DDX52/ROK1 subfamily.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
    • 翻訳後修飾
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 細胞内局在
      Nucleus > nucleolus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ATP dependent RNA helicase ROK1 like antibody
      • ATP-dependent RNA helicase ROK1-like antibody
      • DDX52 antibody
      • DDX52_HUMAN antibody
      • DEAD (Asp-Glu-Ala-Asp) box polypeptide 52 antibody
      • DEAD box protein 52 antibody
      • Human sequence similar to yeast 19 antibody
      • HUSSY19 antibody
      • Probable ATP dependent RNA helicase DDX52 antibody
      • Probable ATP-dependent RNA helicase DDX52 antibody
      • ROK1 antibody
      • ROK1, S. cerevisiae, homolog of antibody
      see all

    画像

    • Immunoprecipitation of DDX52. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Lane 1- DDXR52 was Immunoprecipitated by rabbit anti-DDX52 antibody (ab112027) at 6 µg/mg lysate. Lane 2- DDX52 was also immunoprecipitated by an rabbit anti-DDX52 antibody that recognizes a downstream epitope. Lane 3- Rabbit IgG control Western blot was performed with the same antibody as used in lane 2 at 0.4 mcg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

    プロトコール

    参考文献

    ab112027 has not yet been referenced specifically in any publications.

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