Application
Western blot
Loading amount
0.2 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Purified protein (purified protein)
Specification
purified protein
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: HRP conjugated goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/13000
Detection
Detection method
ECL
Bands
Specific: 68 kDa
Exposure
1 minute(s) and 0 second(s)
Negative control
dephosphorylated p68 protein
Positive control
phosphorylated p68
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MS. Christina George
Verified customer
投稿 Oct 22 2013