Application
Western blot
Sample
Mouse Cell lysate - whole cell (Ovary)
Gel Running Conditions
Reduced Denaturing (8)
Loading amount
30 µg
Specification
Ovary
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/2000
Secondary antibody
Name
Abcam antibody:Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase)
Detection
Detection method
Western Blue Alkaline Phosphatase
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 80 kDa Non-specific: 55, 45, 130, 260 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jul 21 2016