Anti-DDX18 抗体 (ab70526)
Key features and details
- Rabbit polyclonal to DDX18
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-DDX18 antibody
DDX18 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DDX18 -
由来種
Rabbit -
特異性
We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues. -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Rabbit, Cow, Dog, Pig, Chimpanzee, Baboon, Rhesus monkey, Gorilla, Orangutan, Elephant -
免疫原
A region between residues 1 and 50 of human DDX18 (NP_006764.3)
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ポジティブ・コントロール
- Whole cell lysate from HeLa and 293T cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70526の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | ||
IP |
特記事項 |
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WB: 1/2000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
Probable RNA-dependent helicase. -
配列類似性
Belongs to the DEAD box helicase family. DDX18/HAS1 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. - Information by UniProt
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参照データベース
- Entrez Gene: 8886 Human
- Omim: 606355 Human
- SwissProt: Q9NVP1 Human
- Unigene: 728867 Human
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別名
- ATP dependent RNA helicase DDX18 antibody
- ATP-dependent RNA helicase DDX18 antibody
- DDX 18 antibody
see all
画像
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All lanes : Anti-DDX18 antibody (ab70526) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Developed using the ECL technique.
Predicted band size: 75 kDa
Observed band size: 75 kDa
Exposure time: 3 minutes -
Detection of DDX18 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab70526 (Lane 1) and ab70527 (Lane 2) at 3 µg/mg lysate for IP. ab70526 was used at 1 µg/ml for subsequent WB detection. Lane 3 represents control IgG IP.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab70526 は論文での使用が確認できていません。