SureLight® APC Anti-DDDDK tag (Binds to FLAG® tag sequence) 抗体 [M2] (ab72569)
Key features and details
- SureLight® APC Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence)
- Suitable for: FRET, Flow Cyt (Intra)
- Reacts with: Species independent
- Conjugation: SureLight® APC. Ex: 652nm, Em: 657nm
- Isotype: IgG1
製品の概要
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製品名
SureLight® APC Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2]
DDDDK tag (Binds to FLAG® tag sequence) 一次抗体 製品一覧 -
製品の詳細
SureLight® APC Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence) -
由来種
Mouse -
標識
SureLight® APC. Ex: 652nm, Em: 657nm -
アプリケーション
適用あり: FRET, Flow Cyt (Intra)more details -
種交差性
交差種: Species independent -
免疫原
Synthetic peptide corresponding to DDDDK tag (Binds to FLAG® tag sequence).
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特記事項
This antibody has been optimized for High throughput screening (HTS) and fluorescence resonance energy transfer (FRET) assays.
Excitation maximum:652 nm Emission maximum:657.5 nm F:P 1:1.
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.013% Sodium azide
Constituents: 0.1% BSA, 0.87% Sodium chloride, 1.64% Sodium phosphate, 1.71% Sucrose -
Concentration information loading...
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精製度
Size exclusion -
特記事項(精製)
Purified using size exclusion chromatography as well as some proprietary methods for purifying the final materials. -
一次抗体 備考
This antibody has been optimized for High throughput screening (HTS) and fluorescence resonance energy transfer (FRET) assays. -
ポリ/モノ
モノクローナル -
クローン名
M2 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
- DyLight® 650 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab117492)
- DyLight® 550 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab117495)
- DyLight® 488 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab117505)
- PerCP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab117514)
- Europium Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab187914)
- HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)
- PE Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72469)
- Alkaline Phosphatase Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab81655)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab72569の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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FRET |
Use at an assay dependent concentration.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
|
特記事項 |
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FRET
Use at an assay dependent concentration. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
ターゲット情報
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関連性
This is a useful tool for the localisation and characterisation of DDDDK tagged proteins (Binds to FLAG® tag sequence). -
別名
- DDDDK epitope tag antibody
- DDDK antibody
- ddk antibody
see all
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab72569 は 6 報の論文で使用されています。
- Edelstein HI et al. Elucidation and refinement of synthetic receptor mechanisms. Synth Biol (Oxf) 5:ysaa017 (2020). PubMed: 33392392
- Hayashi Y et al. Complex formation of sphingomyelin synthase 1 with glucosylceramide synthase increases sphingomyelin and decreases glucosylceramide levels. J Biol Chem 293:17505-17522 (2018). PubMed: 30242129
- Roy G et al. A novel bicistronic gene design couples stable cell line selection with a fucose switch in a designer CHO host to produce native and afucosylated glycoform antibodies. MAbs 10:416-430 (2018). PubMed: 29400603
- Hayashi Y et al. Carboxyl-terminal Tail-mediated Homodimerizations of Sphingomyelin Synthases Are Responsible for Efficient Export from the Endoplasmic Reticulum. J Biol Chem 292:1122-1141 (2017). PubMed: 27927984
- Kerr SA et al. Computational and Functional Analysis of the Virus-Receptor Interface Reveals Host Range Trade-Offs in New World Arenaviruses. J Virol 89:11643-53 (2015). PubMed: 26355089
- Demogines A et al. Dual host-virus arms races shape an essential housekeeping protein. PLoS Biol 11:e1001571 (2013). Flow Cyt . PubMed: 23723737