Application
Western blot
Sample
Xenopus laevis Cell lysate - whole cell (st II-III oocytes)
Gel Running Conditions
Reduced Denaturing (4-20% precast)
Loading amount
20 cells
Specification
st II-III oocytes
Blocking step
Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/100
Detection
Detection method
SuperSignal Femto ECL
Exposure
5 second(s)
Bands
Specific: 127 kDa Non-specific: 48 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Samantha Jeschonek
Verified customer
投稿 Feb 22 2017