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Products:Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdk Regulators
Overview
Product name
Anti-DBF4 antibody
DBF4 抗体 (5件) 一覧
Description
Rabbit polyclonal to DBF4
Specificity
ab70134 detects endogenous levels of total DBF4 protein.
Tested applications
ICC/IF, WB, ELISAmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide derived from the N terminus of human DBF4.
Positive control
NIH3T3 cell extracts treated with H2O2 (100µM, 30mins), COS7 cell extracts treated with PMA (125mg/ml, 30mins), and NIH3T3 cell extracts treated with both H2O2 (100µM, 30mins) and PMA (125mg/ml, 30mins).
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab70124 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdk Regulators
Applications
Show applications key
Our Abpromise guarantee covers the use of ab70134 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 µg/ml
WB: 1/500 - 1/1000.Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
ELISA
ELISA: 1/10000ELISA: 1/10000
Target
Function
Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.
Tissue specificity
Highly expressed in testis and thymus. Expressed also in most cancer cells lines.
Sequence similarities
Contains 2 BRCT domains.
Contains 1 DBF4-type zinc finger.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9UBU7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Activator of S phase kinase antibody
- ASK antibody
- Chiffon homolog A antibody
see all
Database links
- Entrez Gene: 10926 Human
- Entrez Gene: 27214 Mouse
- Omim: 604281 Human
- SwissProt: Q9UBU7 Human
- SwissProt: Q80YS2 Mouse
- SwissProt: Q9QZ41 Mouse
- Unigene: 485380 Human
- Unigene: 292470 Mouse
Anti-DBF4 antibody images:
Western blot - DBF4 antibody (ab70134)
All lanes : Anti-DBF4 antibody (ab70134) at 1/500 dilution
Lane 1 : NIH3T3 cell extracts
treated with H2O2 (100µM, 30mins) at 5 µg
Lane 2 : COS7 cell extracts
treated with PMA (125mg/ml, 30mins)
Lane 3 : NIH3T3 cell extracts
treated with H2O2 (100µM, 30mins) and PMA (125mg/ml, 30mins) with immunising peptide at 5 µg
Predicted band size : 77 kDa
Observed band size : 77 kDa
Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence-DBF4 antibody(ab70134)
ICC/IF image of ab70134 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70134, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-DBF4 antibody (ab70134)
ab70134 has not yet been referenced specifically in any publications.
Publishing research using ab70134? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"