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Western blot - DBF4 antibody (ab70134)
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Immunocytochemistry/ Immunofluorescence-DBF4 antibody(ab70134)
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Product Name
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DBF4 antibody
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DBF4 抗体 (3件) 一覧
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to DBF4
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Immunogen
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Synthetic peptide derived from the N terminus of human DBF4.
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Reacts with
(species key)
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Hu, Ms
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Specificity
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ab70134 detects endogenous levels of total DBF4 protein.
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Tested applications
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ELISA, ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/10000.
ICC/IF: Use at a concentration of 1 µg/ml.
WB: 1/500 - 1/1000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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NIH3T3 cell extracts treated with H2O2 (100µM, 30mins), COS7 cell extracts treated with PMA (125mg/ml, 30mins), and NIH3T3 cell extracts treated with both H2O2 (100µM, 30mins) and PMA (125mg/ml, 30mins).
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdk Regulators
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Relevance
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DBF4 plays a role as a regulatory subunit for CDC7 which activates its kinase activity. Is required for entry into S phase of the cell cycle. The complex CDC7-DBF4 selectively phosphorylates MCM2 subunit of chromatin-associated MCM complex and then is involved in regulating the initiation of DNA replication during cell cycle.
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DBF4 antibody (ab70134)の別名 |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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ab70124 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Abcam では全ての製品情報を一元管理しており、 DBF4 antibody について私たちが知りうる全ての情報がこのデータシートでご覧いただけます。ご不明な点がありましたら、 お問い合わせください。 |
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See below for DBF4 antibody images, references, products related to ab70134 and other tools.
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DBF4 antibody images:
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 Western blot - DBF4 antibody (ab70134)
All lanes : DBF4 antibody (ab70134) at 1/500 dilution
Lane 1 : NIH3T3 cell extracts
treated with H2O2 (100µM, 30mins) at 5 µg
Lane 2 : COS7 cell extracts
treated with PMA (125mg/ml, 30mins)
Lane 3 : NIH3T3 cell extracts
treated with H2O2 (100µM, 30mins) and PMA (125mg/ml, 30mins) with immunising peptide at 5 µg
Predicted band size : 77 kDa
Observed band size : 77 kDa
Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
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Immunocytochemistry/ Immunofluorescence-DBF4 antibody(ab70134)
ICC/IF image of ab70134 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70134, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Search PubMed (MEDLINE) for references to DBF4
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DBF4 antibody - more information
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Customer reviews (feedback) regarding DBF4 antibody |
Customer FAQs regarding DBF4 antibody |
Protocols for DBF4 antibody |
Price and availability of products related to DBF4 antibody |
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DBF4 antibody - related products:
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DBF4 antibody - other tools:
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Contact Abcam with a Technical Enquiry about DBF4 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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