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Anti-DBF4 antibody (ab70134)

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1 question for ab70134

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Question 1

Tuesday 29-November-2011

Phone call enquiring whether the Western blot on the dateasheet for ab70134 was produced using ectopic expression as the datasheet states the expected size is 77 kDa whilst a report by Jiang et al, EMBO 1999 states that endogenous expression protein size of 100-150 kDa is observed.

ANSWER:

 

Thank you for contacting us in regards to Anti-DBF4 antibody (ab70134).

I have been assured by the source of this antibody that the Western blot performed and displayed on the datasheet was performed with endogenous expression of DBF4 from NIH3T3 and COS7 cells treated with H2O2 and PMA respectively.

As you have pointed out this conflicts with the report by Jiang et al, EMBO 1999.   I am currently looking into other reports of DBF4 expression to see if this difference may be as a result of postranslational modification differences between the two experiments. As soon as I have any further information I will let you know.

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