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Products:Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdk Regulators
Overview
Product name
Anti-DBF4 antibody
DBF4 抗体 (5件) 一覧
Description
Mouse monoclonal to DBF4
Tested applications
IHC-P, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment: NSGAMRIHSK GHFQGGIQVK NEKNRPSLKS LKTDNRPEKS KCKPLWGKVF YLDLPSVTIS EKLQKDIKDL GGRVEEFLSK DISYLISNKK EAKFAQT, corresponding to amino acids 2-99 of Human DBF4
NSGAMRIHSK GHFQGGIQVK NEKNRPSLKS LKTDNRPEKS KCKPLWGKVF YLDLPSVTIS EKLQKDIKDL GGRVEEFLSK DISYLISNKK EAKFAQT
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG1
Light chain type
kappa
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdk Regulators
Applications
Show applications key
Our Abpromise guarantee covers the use of ab56813 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
WB: Use at a concentration of 1-5 µg/ml.
IHC-P: Use at a concentration of 3 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.
Tissue specificity
Highly expressed in testis and thymus. Expressed also in most cancer cells lines.
Sequence similarities
Contains 2 BRCT domains.
Contains 1 DBF4-type zinc finger.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9UBU7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Activator of S phase kinase antibody
- ASK antibody
- Chiffon homolog A antibody
see all
Database links
- Entrez Gene: 10926 Human
- Omim: 604281 Human
- SwissProt: Q9UBU7 Human
- Unigene: 485380 Human
Anti-DBF4 antibody images:
IHC-P - BDF4 antibody (ab56813)
BDF4 antibody (ab56813) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human spleen.
Western blot - DBF4 antibody (ab56813)
Western blot against tagged recombinant protein immunogen using ab56813 BDF4 antibody at 1ug/ml. Predicted band size of immunogen is 36 kDa
References for Anti-DBF4 antibody (ab56813)
ab56813 has not yet been referenced specifically in any publications.
Publishing research using ab56813? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"