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IHC-P - BDF4 antibody (ab56813)
IHC-P - BDF4 antibody (ab56813)
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Western blot - DBF4 antibody (ab56813)
Western blot - DBF4 antibody (ab56813)
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Product Name 

DBF4 antibody

 

DBF4 抗体 (3件) 一覧

Product type 

Primary antibodies

Description 

Mouse monoclonal to DBF4

Immunogen 

Recombinant fragment: NSGAMRIHSK GHFQGGIQVK NEKNRPSLKS LKTDNRPEKS KCKPLWGKVF YLDLPSVTIS EKLQKDIKDL GGRVEEFLSK DISYLISNKK EAKFAQT, corresponding to amino acids 2-99 of Human DBF4
BLAST 'NSGAMRIHSK GHFQGGIQVK NEKNRPSLKS LKTDNRPEKS KCKPLWGKVF YLDLPSVTIS EKLQKDIKDL GGRVEEFLSK DISYLISNKK ' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 
(species key)

Hu

Tested applications 
(see key)

IHC-P, WB


Abreviews 

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Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 1-5 µg/ml.
IHC-P: Use at a concentration of 3 µg/ml.

This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdk Regulators

Relevance 

DBF4 plays a role as a regulatory subunit for CDC7 which activates its kinase activity. Is required for entry into S phase of the cell cycle. The complex CDC7-DBF4 selectively phosphorylates MCM2 subunit of chromatin-associated MCM complex and then is involved in regulating the initiation of DNA replication during cell cycle.

 

DBF4 antibody (ab56813)の別名

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

10926    (Human)

SwissProt

    

Q9UBU7    (Human)

Unigene

    

485380    (Human)

Raised in 

Mouse

Clonality 

Monoclonal

Isotype 

IgG1

Light chain type 

kappa

Purity 

Protein G purified

Storage buffer 

Preservative: None
PBS, pH 7.2

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

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DBF4 antibody images:

Product image  IHC-P - BDF4 antibody (ab56813)

IHC-P - BDF4 antibody (ab56813)

BDF4 antibody (ab56813) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human spleen.

 

Product image  Western blot - DBF4 antibody (ab56813)

Western blot - DBF4 antibody (ab56813)

Western blot against tagged recombinant protein immunogen using ab56813 BDF4 antibody at 1ug/ml. Predicted band size of immunogen is 36 kDa

 
 

Search PubMed (MEDLINE) for references to DBF4

 

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