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Western blot - BDF4 antibody [2047C4a] (ab50624)
![Western blot - BDF4 antibody [2047C4a] (ab50624)](/ps/datasheet/images/ab50624.gif)
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Product Name
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DBF4 antibody [2047C4a]
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DBF4 抗体 (3件) 一覧
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [2047C4a] to DBF4
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Immunogen
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Recombinant human DBF4
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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Dot, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions Dot: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Detects a band of approximately 36 kDa (predicted molecular weight: 77 kDa).
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdk Regulators
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Relevance
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DBF4 plays a role as a regulatory subunit for CDC7 which activates its kinase activity. Is required for entry into S phase of the cell cycle. The complex CDC7-DBF4 selectively phosphorylates MCM2 subunit of chromatin-associated MCM complex and then is involved in regulating the initiation of DNA replication during cell cycle.
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DBF4 antibody [2047C4a] (ab50624)の別名
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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2047C4a
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Isotype
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IgG1
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.05% Sodium Azide Constituents: 1% BSA, PBS (8mM Sodium phosphate, 3mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate) pH 7.4 Material safety datasheets (MSDS) for this product: Sodium Azide MSDS Sodium Phosphate MSDS
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Purification notes
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ab50624 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22 µm membrane.
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Form
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Liquid
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Concentration
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0.100 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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See below for DBF4 antibody [2047C4a] images, references, products related to ab50624 and other tools.
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DBF4 antibody [2047C4a] images:
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Search PubMed (MEDLINE) for references to DBF4
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DBF4 antibody [2047C4a] - more information
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