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Western blot - BDF4 antibody [2047C4a] (ab50624)
Western blot - BDF4 antibody [2047C4a] (ab50624)
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Product Name 

DBF4 antibody [2047C4a]

 

DBF4 抗体 (3件) 一覧

Product type 

Primary antibodies

Description 

Mouse monoclonal [2047C4a] to DBF4

Immunogen 

Recombinant human DBF4

Reacts with 
(species key)

Hu

Tested applications 
(see key)

Dot, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
Dot: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Detects a band of approximately 36 kDa (predicted molecular weight: 77 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdk Regulators

Relevance 

DBF4 plays a role as a regulatory subunit for CDC7 which activates its kinase activity. Is required for entry into S phase of the cell cycle. The complex CDC7-DBF4 selectively phosphorylates MCM2 subunit of chromatin-associated MCM complex and then is involved in regulating the initiation of DNA replication during cell cycle.

 

DBF4 antibody [2047C4a] (ab50624)の別名

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

10926    (Human)

SwissProt

    

Q9UBU7    (Human)

Unigene

    

485380    (Human)

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

2047C4a

Isotype 

IgG1

Purity 

Protein G purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS (8mM Sodium phosphate, 3mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate) pH 7.4

Material safety datasheets (MSDS) for this product:
Sodium Azide MSDS
Sodium Phosphate MSDS

Purification notes 

ab50624 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22 µm membrane.

Form 

Liquid

Concentration 

0.100 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

 

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See below for DBF4 antibody [2047C4a] images, references, products related to ab50624 and other tools.

 

DBF4 antibody [2047C4a] images:

Product image  Western blot - BDF4 antibody [2047C4a] (ab50624)

Western blot - BDF4 antibody [2047C4a] (ab50624)

DBF4 antibody [2047C4a] (ab50624) + immunized recombinant protein

Predicted band size : 77 kDa
Observed band size : 36 kDa (why is the actual band size different from the predicted?)

 
 

Search PubMed (MEDLINE) for references to DBF4

 

DBF4 antibody [2047C4a] - more information

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Price and availability of products related to DBF4 antibody [2047C4a]

 

DBF4 antibody [2047C4a] - related products:

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