Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.01M citric acid/sodium citrate
Sample
Mouse Tissue sections (adult testes)
Specification
adult testes
Permeabilization
No
Fixative
4%PFA
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti Rabbit IgG(H+L)/Biotin IgG(H+L) ZSGB-Bio
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Abcam response
Thank you for your valuable feedback. This customer was contacted and was given protocol tips to reduce the high background. We have suggested diluting this antibody to 0.5 - 5 ´g/ml and changing the blocking conditions. In addition, this customer obtained good results with this antibody on frozen tissues so the problem may also be from the fixation and antigen retrieval methods.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Qing Wen
Verified customer
投稿 May 06 2014