Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Embryonic day 13 gonads)
Specification
Embryonic day 13 gonads
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Triton
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Other product details
Dilution
1/100
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: Alexa 568 anti rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Dilution
1/300
Additional data
Additional Notes
Anti-Dazl antibody (red) shows specific cytoplasmic staining only in primordial germ cells (Oct4, green), and not in surrounding somatic cells (DAPI, blue). This is as expected. However, it also stains non-specifically the membrane of both primordial germ cells and surrounding somatic cells.
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投稿 Jan 22 2013