Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Testis, adult)
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Specification
Testis, adult
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C
Dilution
1/250
Secondary antibody
Name
Abcam antibody:Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed
Additional data
Additional Notes
DAZL has been shown in the literature to be localized to the cytoplasm of spermatagonia and spermatocytes. This antibody (or at least this lot) stains the nuclei of spermatids.
Abcam response
Thank you for sharing this data with us. ab108372 has been tested in IHC-P in our own lab and is not suitable for this application. We would recommend ab34139 to all researchers wishing to do IHC-Fr for DAZL. Abcam would welcome feedback from other researchers using these antibodies.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Bryan Niedenberger
Verified customer
投稿 Mar 11 2016