製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to DAZAP1
  • アプリケーション
    適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    A region within synthetic peptide: QAAPDMSKPP TAQPDFPYGQ YGLGSYSPAP PGCGPHFVYS LMVRLSSDVA, corresponding to amino acids 240-289 of Human DAZAP1

  • ポジティブ・コントロール
    • HepG2 cell lysate, Human kidney.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab51010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 43 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA 1/312500.

Titre using peptide based assay 1/312500.

ターゲット情報

  • 機能
    RNA-binding protein, which may be required during spermatogenesis.
  • 組織特異性
    Mainly expressed in testis. Expressed to a lower level in thymus. Weakly or not expressed in heart, liver, brain, placenta, lung, skeletal muscle, kidney and pancreas.
  • 配列類似性
    Contains 2 RRM (RNA recognition motif) domains.
  • 翻訳後修飾
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Cytoplasm. Nucleus. Predominantly cytoplasmic (By similarity). Nuclear at some stages of spermatozoides development. In midpachytene spermatocytes, it is localized in both the cytoplasm and the nuclei and is clearly excluded from the sex vesicles. In round spermatids, it localizes mainly in the nuclei, whereas in elongated spermatids, it localizes to the cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DAZ associated protein 1 antibody
    • DAZ-associated protein 1 antibody
    • Dazap1 antibody
    • DAZP1_HUMAN antibody
    • Deleted in azoospermia associated protein 1 antibody
    • Deleted in azoospermia-associated protein 1 antibody
    see all

画像

  • Lane 1 : Marker
    Lane 2 : Anti-DAZAP1 antibody (ab51010) at 0.5 µg/ml

    Lane 1 : As above
    Lane 2 : HepG2 cell lysate at 10 µg with skim milk/ PBS at 5 %

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 43 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
  • Ab51010 at 4.0 µg/ml staining epithelial cells of renal tubule from human kidney; paraffin embedded; magnification 400x.

参考文献

This product has been referenced in:
  • Hayakawa H  et al. Human proteins that specifically bind to 8-oxoguanine-containing RNA and their responses to oxidative stress. Biochem Biophys Res Commun 403:220-4 (2010). WB ; Human . Read more (PubMed: 21073862) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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