Anti-DAP3 抗体 (ab227257)
Key features and details
- Rabbit polyclonal to DAP3
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-DAP3 antibody
DAP3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DAP3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant fragment within Human DAP3 (internal sequence). The exact sequence is proprietary.
Database link: P51398 -
ポジティブ・コントロール
- IHC-P: MDA-MB-231 xenograft tissue. WB: HepG2 whole cell lysate. ICC/IF: HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab227257の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 46 kDa.
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ICC/IF |
1/100 - 1/1000.
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IHC-P |
1/100 - 1/1000.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 46 kDa. |
ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. |
ターゲット情報
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機能
Involved in mediating interferon-gamma-induced cell death. -
組織特異性
Ubiquitous. -
細胞内局在
Mitochondrion. - Information by UniProt
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参照データベース
- Entrez Gene: 7818 Human
- Entrez Gene: 65111 Mouse
- Omim: 602074 Human
- SwissProt: P51398 Human
- SwissProt: Q9ER88 Mouse
- Unigene: 516746 Human
- Unigene: 29028 Mouse
- Unigene: 400397 Mouse
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別名
- 28S ribosomal protein S29 antibody
- 28S ribosomal protein S29 mitochondrial antibody
- bMRP10 antibody
see all
画像
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Anti-DAP3 antibody (ab227257) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 46 kDa10% SDS-PAGE gel.
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Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for DAP3 (green) using ab227257 at 1/200 dilution in ICC/IF. Nuclear Counterstain: Hoechst 33342 (blue).
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Paraffin-embedded MDA-MB-231 xenograft tissue stained for DAP3 with ab227257 at 1/250 dilution in immunohistochemical analysis.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab227257 は論文での使用が確認できていません。