Anti-DAP1 抗体 [E59] (ab32056)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E59] to DAP1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-DAP1 antibody [E59]
DAP1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [E59] to DAP1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details
適用なし: Flow Cyt -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide within Human DAP1 aa 50-150 (C terminal). The exact sequence is proprietary.
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ポジティブ・コントロール
- NIH3T3 cell lysate, Hela cell lysate and human stomach carcinoma
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
E59 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32056の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/10000. Detects a band of approximately 11 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/250.
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特記事項 |
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WB
1/10000. Detects a band of approximately 11 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/250. |
ターゲット情報
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機能
Involved in mediating interferon-gamma-induced cell death. - Information by UniProt
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参照データベース
- Entrez Gene: 1611 Human
- Entrez Gene: 223453 Mouse
- Omim: 600954 Human
- SwissProt: P51397 Human
- SwissProt: Q91XC8 Mouse
- Unigene: 75189 Human
- Unigene: 222867 Mouse
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別名
- DAP 1 antibody
- Dap antibody
- DAP-1 antibody
see all
画像
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Immunofluorescent analysis of DAP1 expression in HeLa cells using 1/250 ab32056.
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Anti-DAP1 antibody [E59] (ab32056) at 1/10000 dilution + NIH3T3 cell lysate
Observed band size: 11 kDa why is the actual band size different from the predicted?
Additional bands at: 23 kDa. We are unsure as to the identity of these extra bands. -
ab32056, at a 1/100 dilution, staining human stomach carcinoma by immunohistochemistry, Paraffin embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab32056 は 1 報の論文で使用されています。
- Raj P et al. Deep sequencing reveals a DAP1 regulatory haplotype that potentiates autoimmunity in systemic lupus erythematosus. Genome Biol 21:281 (2020). PubMed: 33213505