Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10)
Sample
Monkey Cell lysate - other (COS 7)
Specification
COS 7
Treatment
TRANSFECT DAGLA
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: HRP ANTI GOAT
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000
Detection
Detection method
ECL
Bands
Specific: 115 kDa Non-specific: ~100 kDa
Exposure
5 minute(s) and 0 second(s)
Negative control
no DAGLA transfect
Positive control
DAGLA transfect
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Sep 30 2013