Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Monkey Tissue sections (Marmoset cerebellum)
Specification
Marmoset cerebellum
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Other product details
Dilution
1/5000
Incubation time
2 hour(s) and 0 minute(s) · Diluent: TBS/BSA/azide pH7.5
Secondary antibody
Name
Non-Abcam antibody was used: anti sheep IgG
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Biotin
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Additional Notes
I obtain a very similar positivity of Purkinje cell secondary dendrites in Marmoset, Rat and Mouse cerebellums. The five-fold dilution factor of the primary antibody ( I used 1/1000 in my rat and mouse Abreviews) is achieved because I used a commercial DAB kit formulation that is more sensitive than my in-house DAB formulation.
NB: the anti sheep biotinylated secondary has a 50% cross-reactivity with Goat IgGs and is thus suitable for secondary detection of Goat IgG primary Abs.
NB: the anti sheep biotinylated secondary has a 50% cross-reactivity with Goat IgGs and is thus suitable for secondary detection of Goat IgG primary Abs.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
投稿 Dec 15 2009