Application
Western blot
Sample
Human Tissue lysate - whole (skin)
Loading amount
15 µg
Specification
skin
Gel Running Conditions
Non-reduced Denaturing (15% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Other product details
Dilution
1/200
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBST plus 1% BSA
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 555 donkey anti-rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Dilution
1/3000
Detection
Detection method
fluorescent
Exposure
2 second(s)
Bands
Specific: 25 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 24 2009