Anti-Cytomegalovirus Glycoprotein B 抗体 [HCMV37] (ab54023)
Key features and details
- Mouse monoclonal [HCMV37] to Cytomegalovirus Glycoprotein B
- Suitable for: ELISA, ICC/IF, IHC-Fr, Neutralising
- Reacts with: Human cytomegalovirus
- Isotype: IgG2a
Related conjugates and formulations
製品の概要
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製品名
Anti-Cytomegalovirus Glycoprotein B antibody [HCMV37]
Cytomegalovirus Glycoprotein B 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [HCMV37] to Cytomegalovirus Glycoprotein B -
由来種
Mouse -
特異性
This antibody is specific for Cytomegalovirus glycoprotein B late neutralizing antigen. Reacts with MRC-5 infected cells but not uninfected cells. -
アプリケーション
適用あり: ELISA, ICC/IF, IHC-Fr, Neutralisingmore details -
種交差性
交差種: Human cytomegalovirus -
免疫原
Human CMV extracts
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ポジティブ・コントロール
- ICC/IF: monocyte-derived dendritic cells infected with Human cytomegalovirus
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
HCMV37 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab54023の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA | ||
ICC/IF | ||
IHC-Fr | ||
Neutralising |
特記事項 |
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ICC/IF: Use at an assay dependent dilution (CMV infected cells).
IHC-Fr: Use at an assay dependent dilution (CMV infected cells).
Neut: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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関連性
Human cytomegalovirus (HCMV) glycoprotein B (gB) is an abundant virion envelope protein that has been shown to be essential for the infectivity of HCMV. HCMV gB is also one of the most immunogenic virus-encoded proteins, and a significant fraction of virus neutralizing antibodies are directed at gB. -
細胞内局在
Virus envelope. -
参照データベース
- Entrez Gene: 3077424 Human cytomegalovirus
- SwissProt: P06473 Human cytomegalovirus
- SwissProt: P13201 Human cytomegalovirus
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別名
- CMV glycoprotein B antibody
- Glycoprotein B antibody
- HCMV antibody
see all
画像
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ab54023, staining Cytomegalovirus Glycoprotein B in monocyte-derived dendritic cells infected with Human cytomegalovirus (VHL/E strain), cultured for 3 hours (left panels) and 24 hours (right panels), by Immunocytochemistry/ Immunofluorescence.
Cells were fixed with paraformaldehyde and permeabilized with 0.2% Triton X-100 in PBS for 10 minutes at room temperature. Samples were incubated with primary antibody and an AlexaFluor®568-conjugated goat anti-mouse monoclonal IgG was used as the secondary antibody.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (6)
ab54023 は 6 報の論文で使用されています。
- Alba-Patiño A et al. Nanoparticle Reservoirs for Paper-Only Immunosensors. ACS Sens 5:147-153 (2020). PubMed: 31850749
- Ulasov IV et al. CMV70-3P miRNA contributes to the CMV mediated glioma stemness and represents a target for glioma experimental therapy. Oncotarget 8:25989-25999 (2017). PubMed: 27517625
- Wang B et al. Viperin is induced following toll-like receptor 3 (TLR3) ligation and has a virus-responsive function in human trophoblast cells. Placenta 36:667-73 (2015). PubMed: 25814471
- Kirchmeier M et al. Enveloped virus-like particle expression of human cytomegalovirus glycoprotein B antigen induces antibodies with potent and broad neutralizing activity. Clin Vaccine Immunol 21:174-80 (2014). Neutralising . PubMed: 24334684
- Haspot F et al. Human cytomegalovirus entry into dendritic cells occurs via a macropinocytosis-like pathway in a pH-independent and cholesterol-dependent manner. PLoS One 7:e34795 (2012). ICC/IF . PubMed: 22496863
- Cranage MP et al. Identification of the human cytomegalovirus glycoprotein B gene and induction of neutralizing antibodies via its expression in recombinant vaccinia virus. EMBO J 5:3057-63 (1986). PubMed: 3024973