Application
Immunocytochemistry
Sample
Human Cultured Cells (primary cells from human skin (keratinocytes / fib)
Specification
primary cells from human skin (keratinocytes / fib
Fixative
EtOH:Acetone (1:1)
Permeabilization
Yes - Triton-X-100 / 0,1% / 5 min
Blocking step
(agent) for 5 minute(s) · Concentration: 100% · Temperature: 21°C
Other product details
Dilution
1/100
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: FBS Stain-Buffer (BD Pharmingen)
Secondary antibody
Name
Non-Abcam antibody was used: Dako EnVision, Peroxidase/DAB+, rabbit / mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Peroxidase Blocking Solution (DAKO / 3% H2O2) before staining
DAKO Envision with DAB as substrate
Staining of negative cells with Mayer´s hematoxilin
Corresponding Isotype control IgG1 (DAKO) as negative control in same dilution as primary antibody. Concentration n.d. by abcam!!
Discount code: 51922
DAKO Envision with DAB as substrate
Staining of negative cells with Mayer´s hematoxilin
Corresponding Isotype control IgG1 (DAKO) as negative control in same dilution as primary antibody. Concentration n.d. by abcam!!
Discount code: 51922
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jul 07 2010