Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Bos taurus Tissue sections (Airway epithelium (basal epithelial cells))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9.0
Permeabilization
No
Specification
Airway epithelium (basal epithelial cells)
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Dilution
1/400
Secondary antibody
Dilution
1/500
Name
Abcam antibody:Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) preadsorbed
Additional data
Additional Notes
Red, Cytokeratin 5 Green, Ki67 Blue, DAPI (nuclei)
Airway basal stem/progenitor epithelial cells were positively stained with anti-cytokeratin 5 monoclonal rabbit antibody. Some of the CK5-positive cells expressed Ki67, a marker of mitosis.
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投稿 Nov 26 2015