Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Sample
Human Cell (HCC1143 cell lines)
Specification
HCC1143 cell lines
Permeabilization
Yes - 0.3% Triton X-100 in TBS
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.3% Triton X-100 in TBS with 5% FBS
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: Life Tech - Alexa Fluor 488 anti Rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 25 2015