Anti-Cytokeratin 18 抗体 [RGE53] (ab9217)
Key features and details
- Mouse monoclonal [RGE53] to Cytokeratin 18
- Suitable for: Flow Cyt, IHC-Fr, WB
- Reacts with: Human, Pig, Zebrafish
- Isotype: IgG1
製品の概要
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製品名
Anti-Cytokeratin 18 antibody [RGE53]
Cytokeratin 18 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [RGE53] to Cytokeratin 18 -
由来種
Mouse -
特異性
This antibody reacts exclusively with cytokeratin 18 which is present in glandular epithelial cells of the digestive, respiratory, and urogenital tracts, endocrine and exocrine cells and mesothelial cells, as well as adenocarcinomas originating from them -
アプリケーション
適用あり: Flow Cyt, IHC-Fr, WBmore details -
種交差性
交差種: Human, Pig, Zebrafish
交差が予測される動物種: Mouse, Rat, Rabbit, Chicken, Hamster, Dog -
免疫原
Full length protein corresponding to Human Cytokeratin 18. Cytoskeletal preparation of HeLa cells.
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ポジティブ・コントロール
- This antibody gave a positive signal in HeLa and MCF7 whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
RGE53 -
ミエローマ
unknown -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9217の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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IHC-Fr |
1/100 - 1/200.
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WB |
Use a concentration of 5 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
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特記事項 |
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Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-Fr
1/100 - 1/200. |
WB
Use a concentration of 5 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). |
ターゲット情報
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機能
Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. -
組織特異性
Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma. -
関連疾患
Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600]. -
配列類似性
Belongs to the intermediate filament family. -
翻訳後修飾
Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues. -
細胞内局在
Cytoplasm > perinuclear region. - Information by UniProt
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参照データベース
- Entrez Gene: 477601 Dog
- Entrez Gene: 3875 Human
- Entrez Gene: 16668 Mouse
- Entrez Gene: 100126286 Pig
- Entrez Gene: 294853 Rat
- Entrez Gene: 352912 Zebrafish
- Omim: 148070 Human
- SwissProt: P05783 Human
see all -
別名
- Cell proliferation inducing gene 46 protein antibody
- Cell proliferation inducing protein 46 antibody
- Cell proliferation-inducing gene 46 protein antibody
see all
画像
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All lanes : Anti-Cytokeratin 18 antibody [RGE53] (ab9217) at 5 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 30 seconds -
Immunohistochemistry on frozen section of human kidney epithelium.
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Immunofluorescence staining images of 9 day old zebrafish embryos.
ab9217 reacts with with intestinal epithelium and epidermis. Frozen sample treated with Acetone:Methanol 1:1, antibody diluted 1/100 and incubated for 45 minutes at room temperature.0 -
immunofluorescence staining of epithelial tissues in a 2 days old zebrafish embryo.
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Immunofluorescence staining of 1 month old zebrafish embryo.
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Immunofluorescence staining images of 9 day old zebrafish embryos.
ab9217 reacts with intestinal epithelium and epidermis. Frozen sample treated with Acetone:Methanol 1:1, antibody diluted 1/100 and incubated for 45 minutes at room temperature. -
Immunohistochemistry on frozen section of human kidney epithelium
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Immunohistochemistry on frozen section of human colon
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Immunohistochemistry on frozen section of swine liver hepatocytes
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Overlay histogram showing MCF7 cells stained with ab9217 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9217, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab9217 は 7 報の論文で使用されています。
- Montalbano M et al. Modeling of Hepatocytes Proliferation Isolated from Proximal and Distal Zones from Human Hepatocellular Carcinoma Lesion. PLoS One 11:e0153613 (2016). WB, IHC-P ; Human . PubMed: 27074018
- Montalbano M et al. Transformation of primary human hepatocytes in hepatocellular carcinoma. Int J Oncol 48:1205-17 (2016). PubMed: 26717856
- Xu J et al. Tbx18 is essential for normal development of vasculature network and glomerular mesangium in the mammalian kidney. Dev Biol 391:17-31 (2014). PubMed: 24727670
- Liu J et al. Cell-to-cell contact induces human adipose tissue-derived stromal cells to differentiate into urothelium-like cells in vitro. Biochem Biophys Res Commun 390:931-6 (2009). ICC/IF ; Human . PubMed: 19852942
- Ramaekers F et al. Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas. Am J Pathol 136:641-55 (1990). PubMed: 1690512
- Puts JJ et al. Expression of cytokeratins in early neoplastic epithelial lesions of the uterine cervix. Int J Gynecol Pathol 4:300-13 (1985). PubMed: 2417968
- Ramaekers F et al. Monoclonal antibody to keratin filaments, specific for glandular epithelia and their tumors. Use in surgical pathology. Lab Invest 49:353-61 (1983). PubMed: 6193333