Application
Western blot
Sample
Human Cell lysate - whole cell (pancreatic cancer cell line)
Loading amount
20 µg
Specification
pancreatic cancer cell line
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 5% milk in TBS/Tween
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-mouse HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 45 kDa
Additional data
PubMed ID
17875703: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Hannah Whiteman
Verified customer
投稿 Jul 04 2008