Application
Flow Cytometry
Sample
Human Cell (keratinocytes)
Specification
keratinocytes
Preparation
Cell harvesting/tissue preparation method: cells were isolated from skin biopsie followed by cell culture
Sample buffer: PBS/10% human IgG
Sample buffer: PBS/10% human IgG
Fixation
Paraformaldehyde
Permeabilization
Yes - 0,1% Tritonx-100/PBS
Gating Strategy
no gate
Other product details
Dilution
1/100
Incubation time
45 minute(s) · Temperature: 4°C · Diluent: PBS/10% human IgG
Secondary antibody
Name
Non-Abcam antibody was used: goat anti rabbit IgG FITC
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Dilution
1/100
Additional data
Additional Notes
keratinocytes:
1 = isotype control
2 = keratinocytes
1 = isotype control
2 = keratinocytes
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 21 2013