Application
Western blot
Loading amount
35 µg
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Sample
Rat Cell lysate - other (Rat hepatocytes plasma membrane fraction)
Specification
Rat hepatocytes plasma membrane fraction
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA in PBS
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: Donkey-anti-Rb
Host species: Donkey
Clonality: Polyclonal
Conjugation: Agarose
Host species: Donkey
Clonality: Polyclonal
Conjugation: Agarose
Dilution
1/10000
Detection
Detection method
ECL+
Bands
Specific: Between 72 and 55 kD kDa Non-specific: Betwwen 55 and 36 kD kDa
Exposure
1 minute(s) and 0 second(s)
Additional data
Additional Notes
MW of Rat Cytokeratin 1 is 64 kDa. Specific band is detected between 72 and 55 kDa.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Armen Petrosyan
Verified customer
投稿 Oct 28 2014