Application
Western blot
Sample
Mouse Tissue lysate - whole (skeletal muscle)
Gel Running Conditions
Reduced Denaturing (15% gel)
Loading amount
50 µg
Specification
skeletal muscle
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Dilution
1/4000
Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
30 second(s)
Bands
Specific: 22 kDa Non-specific: 34 kDa
Positive control
Lysate from mouse liver
Additional data
Additional Notes
Representative western blot of p22phox protein in lysate from single isolated FDB muscle fibers and whole GTN muscle.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. G.K Sakellariou
Verified customer
投稿 Sep 28 2012