Application
Western blot
Sample
Human Tissue lysate - whole (endometrial cancer and adjacent control endometriu)
Loading amount
60 µg
Specification
endometrial cancer and adjacent control endometriu
Gel Running Conditions
Reduced Denaturing (10 % Tris-Glicine gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% milk in TTBS
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-mouse IgG + IgM (H
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: cca 18 kDa
Additional data
PubMed ID
20887769: View
Additional Notes
C... adjacent control endometrium
T... endometrial cancer
T... endometrial cancer
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Feb 07 2011