Application
Western blot
Sample
Mouse Tissue lysate - whole (skin)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
skin
Blocking step
licor fluorescent blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 24°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer
Dilution
1/300
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: licor donkey anti rabbit irdye 680rd
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 680RD
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 680RD
Detection
Detection method
odyssey fluorescent scanner
Exposure
20 minute(s) and 0 second(s)
Bands
Specific: 18 kDa Non-specific: 50 kDa
Additional data
Additional Notes
Precision Plus Protein™ Kaleidoscope™ Prestained Protein Standards was used for the ladder
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Nov 23 2016