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Products:Microbiology >> Interspecies Interaction >> Host Virus Interaction
Overview
Product name
Anti-Cyclin T1 antibody
Cyclin T1 抗体 (4件) 一覧
Description
Rabbit polyclonal to Cyclin T1
Specificity
Antiserum will specifically react with an 85 kDa cyclin T1 protein. No reaction was observed against other related cyclins.
Tested applications
WB, IP, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Fusion protein (Human).
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
serum with 0.01% sodium azide
Purity
Whole antiserum
Purification notes
This product was prepared from monospecific antiserum by delipidation and defibrination.
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Immune System Diseases >> Antiviral Signaling >> HIV-related
Epigenetics and Nuclear Signaling >> Cell cycle >> Cyclins >> Other Cyclins
Cell Biology >> Cell Cycle >> Cyclins >> Other Cyclins
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2098 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 90 kDa (predicted molecular weight: 81 kDa).
IP: Use at an assay dependent dilution.
ELISA
ELISA: 1/500 - 1/2000.ELISA: 1/500 - 1/2000.
IHC-P: 1/200 - 1/1000.
Target
Function
Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes.
Tissue specificity
Ubiquitously expressed.
Sequence similarities
Belongs to the cyclin family. Cyclin C subfamily.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO60563
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CCN T1 antibody
- CCNT antibody
- CCNT 1 antibody
see all
Database links
- Entrez Gene: 904 Human
- Entrez Gene: 12455 Mouse
- Entrez Gene: 315291 Rat
- Omim: 143055 Human
- SwissProt: O60563 Human
- SwissProt: Q9QWV9 Mouse
- Unigene: 279906 Human
- Unigene: 29941 Mouse
see all
Anti-Cyclin T1 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Cyclin T1 antibody(ab2098)
ab2098 (2µg/ml) staining Cyclin T1 in human lymph node using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Western blot - Cyclin T1 antibody (ab2098)
Anti-Cyclin T1 antibody (ab2098) at 1/10000 dilution + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 81 kDa
Observed band size : 90 kDa (why is the actual band size different from the predicted?)
Additional bands at : 100 kDa,48 kDa,78 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 16 minutes
The 90 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to Cyclin T1.
References for Anti-Cyclin T1 antibody (ab2098)
This product has been referenced in:
- Chen Het al. CD4+ T cells from elite controllers resist HIV-1 infection by selective upregulation of p21. J Clin Invest 121:1549-60 (2011). IP; Human.Read more (PubMed: 21403397) »
- Lin Cet al. AFF4, a component of the ELL/P-TEFb elongation complex and a shared subunit of MLL chimeras, can link transcription elongation to leukemia. Mol Cell 37:429-37 (2010). WB, IP.Read more (PubMed: 20159561) »
See all 7 publications for this product
Publishing research using ab2098? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"