Application
Western blot
Sample
Mouse Cell lysate - whole cell (pre-B cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
pre-B cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: anit-Rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
chemiluminescent
Negative control
Ccnd3-mutant vector with mutation at T283 site.
Exposure
10 minute(s) and 0 second(s)
Positive control
Ccnd3 vector
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Nov 20 2013