Application
Western blot
Sample
Human Cell lysate - whole cell (SW480 cells)
Loading amount
25 µg
Specification
SW480 cells
Gel Running Conditions
Reduced Denaturing (4-12%)
Other product details
Dilution
1/200
Incubation time
30 minute(s) · Temperature: 25°C · Diluent: Fast Western Antibody Diluent
Secondary antibody
Name
Non-Abcam antibody was used: Fast Western Optimized HRP Reagent
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 33 kDa
Additional data
Additional Notes
Lane 1 - SW480
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 24 2013