Application
Western blot
Sample
Xenopus laevis Cell lysate - whole cell (early embryos before MBT)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
1 cells
Specification
early embryos before MBT
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-T with 3%BSA
Concentration
2 µg/ml
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Anti-Mouse IgG
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: ~48 kDa Non-specific: 130, 41, 32, 60 kDa
Positive control
unfertilized egg
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Jolanta Kisielewska
Verified customer
投稿 Jul 23 2009