Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Permeabilization
No
Fixative
Methanol
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Dilution
1/500
Secondary antibody
Name
Abcam antibody:Goat Anti-Rabbit IgG H&L (Alexa Fluor 488) preadsorbed
Additional data
Additional Notes
Asynchronous cells were MeOH-fixed, immunofluorescently labeled and counterstained with DAPI. This antibody produces a strong focal staining pattern throughout that is enriched within the nuclei of a subset of interphase cells, which is in agreement with its role in cell cycle regulation. Paraformaldehyde-fixed samples were evaluated and failed to produce the expected staining pattern.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kirk Mcmanus
Verified customer
投稿 Aug 20 2014