Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium citrate pH 6.0
Sample
Mouse Tissue sections (Testes)
Specification
Testes
Blocking step
Serum as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-Tween 20
Dilution
1/300
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit Alex 568
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor 568
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor 568
Dilution
1/1000
Additional data
Additional Notes
The antibody is not specific to CCNA1 protein. There is a strong signal in tissue section from mutant mouse.
Abcam response
We have yet to validate this antibody in mouse tissues. We would welcome any additional data showing the use of this antibody in mouse tissues.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Manju Sharma
Verified customer
投稿 Mar 19 2015