Anti-Cy3 / Cy5 抗体 [CY96] (ab52060)
Key features and details
- Mouse monoclonal [CY96] to Cy3 / Cy5
- Suitable for: Dot blot, ELISA, IP, ICC, ICC/IF
- Reacts with: Species independent
- Isotype: IgG2a
製品の概要
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製品名
Anti-Cy3 / Cy5 antibody [CY96] -
製品の詳細
Mouse monoclonal [CY96] to Cy3 / Cy5 -
由来種
Mouse -
アプリケーション
適用あり: Dot blot, ELISA, IP, ICC, ICC/IFmore details -
種交差性
交差種: Species independent -
免疫原
A mixture of proteins labelled with Cy3 or Cy5.
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特記事項
Monoclonal antibodies to Cy3 and/or Cy5 are an important tool for Cy3/Cy5 signal amplification in assays where biomolecules are labelled with Cy3 or Cy5.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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特記事項(精製)
Purified Mouse Immunoglobulin -
一次抗体 備考
Monoclonal antibodies to Cy3 and/or Cy5 are an important tool for Cy3/Cy5 signal amplification in assays where biomolecules are labelled with Cy3 or Cy5. -
ポリ/モノ
モノクローナル -
クローン名
CY96 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab52060の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Dot blot |
Use a concentration of 1 - 2 µg/ml.
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ELISA |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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ICC |
Use at an assay dependent concentration.
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ICC/IF | (1) |
Use at an assay dependent concentration.
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特記事項 |
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Dot blot
Use a concentration of 1 - 2 µg/ml. |
ELISA
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
ICC
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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関連性
Cy3 and Cy5 are reactive watersoluble fluorescent dyes of the cyanine dye family used for labelling proteins, peptides, DNA, RNA, and other biomolecules. They are usually synthesized with reactive groups on either one or both of the nitrogen side chains so that they can be chemically cross-linked to nucleic acids or protein molecules. Thay are small, pH insensitive (between pH 3 and pH 10), soluble in aqueous solution and have tolerance to DMSO. They are more photostable than fluorescein, have high molar extinction coefficients and favorable quantum yields. They are used in many different biological assays, including DNA microarrays, protein microarrays, two-dimensional protein analysis, fluorescence resonance energy transfer, and immunocytochemistry. Cy3 is excited maximally at 550 nm and emits maximally at 570 nm, in the green part of the spectrum. Cy5 is excited maximally at 649 nm and emits maximally at 670 nm, in the red part of the spectrum.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab52060 は 6 報の論文で使用されています。
- Wang C et al. RasGRP1 promotes the acute inflammatory response and restricts inflammation-associated cancer cell growth. Nat Commun 13:7001 (2022). PubMed: 36385095
- Rodriguez FJ et al. Alternative lengthening of telomeres, ATRX loss and H3-K27M mutations in histologically defined pilocytic astrocytoma with anaplasia. Brain Pathol 29:126-140 (2019). PubMed: 30192422
- Rodriguez FJ et al. Telomere alterations in neurofibromatosis type 1-associated solid tumors. Acta Neuropathol Commun 7:139 (2019). PubMed: 31462295
- Palsgrove DN et al. Subependymal giant cell astrocytoma-like astrocytoma: a neoplasm with a distinct phenotype and frequent neurofibromatosis type-1-association. Mod Pathol N/A:N/A (2018). PubMed: 29973652
- Osterwald S et al. PML induces compaction, TRF2 depletion and DNA damage signaling at telomeres and promotes their alternative lengthening. J Cell Sci 128:1887-900 (2015). PubMed: 25908860
- Jeffery R et al. Identifying the origin and phenotype of cells in tumor xenografts. Methods Mol Biol 1070:235-45 (2014). PubMed: 24092445