Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Head and Neck Squamous Cell Carcinoma)
Specification
Head and Neck Squamous Cell Carcinoma
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
Yes - PBST
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/300
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: Vector Lab Goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/1000
Additional data
Additional Notes
For IHC-P application the other abcam antibody (Anti-Ctip2 antibody [25B6] - ChIP Grade (ab18465) ) seem to work better for us.
Abcam response
Thank you for submitting this review. The lot number used for this staining dates back to about 4 years ago. Although if the antibody has been stored as instructed on the datasheet the antibody should have retained its activity, it may be that over time it has been reduced. It may be worth investing in a newer vial to obtain clearer staining.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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投稿 Oct 05 2012